PRODUCT
Single Cell RNA Sequencing Kit
Enables single cell RNA sequencing across dozens of samples with 125,000 cells or nuclei in parallel.
Description
The ScaleBio Single Cell RNA Sequencing Kit enables the production of high-quality single-cell transcriptomic libraries. Our upfront cell fixation step allows you to take a flexible approach to sample collection, whether from multiple sites or multiple timepoints. This approachable 2-day workflow let's you profile up to 125,000 cells or nuclei as well as multiplex up to 96 samples in a single experiment.
Applications
Cell Atlasing
Disease Research
Large Patient Cohort Analysis
Biomarker Discovery
Workflow
Single cell or nuclei suspensions are prepared and fixed using the ScaleBio Sample Fixation Kit. Samples can be used immediately or stored. Cells or nuclei are then barcoded through three split-pool indexing rounds to produce a sequence-ready library. Data is analyzed with the ScaleBio RNA pipeline.
Unravel sample heterogeneity to discover biology
Over 125,000 PBMCs were profiled to explore cellular heterogeneity within a complex sample type. The UMAP clustering data demonstrates the cell type and cell state heterogeneity of the sample, all easily identified with ScaleBio’s sensitive single cell combinatorial indexing chemistry.
Specifications
Sample Compatibility
Single cell or nuclei suspensions
Multiplexing
1 - 96 samples per run
Throughput
125,000 cells or nuclei
Multiple rate
<5%
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Frequently asked questions
Together with our customers, we have demonstrated performance of our Single Cell RNA Sequencing Kit across a broad range of cell types, including but not limited to: tissue-dissociated cells, nuclei isolated from frozen and fresh tissues, PBMCs, and cell lines.
After fixation, samples can be stored immediately at -80C or transported on dry ice and then stored at -80C. Current storage times are up to 1 month. This is expected to be extended after further validation studies.
The first distribution of cells into the Reverse Transcription (RT) plate assigns a well-specific barcode that is used to identify the sample(s) during data analysis. Samples can be distributed across multiple wells for deeper sample profiling, or each well can contain an individual sample for maximum sample throughput.
ScaleBio provides a downstream data analysis pipeline, which is compatible with sequencing instrument output files. The pipeline will de-multiplex samples, generate a cell-gene matrix, as well as provide a data quality metrics summary to assess run performance.
ScaleBio RNA libraries have been demonstrated with MiSeq, NextSeq and NovaSeq instruments.
Want to learn more about our Single Cell RNA Sequencing Kit?
Reach out to our Team for more information or to request a quote.
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